Target
Human DAPP1 / BAM32
Synonyms
DAPP1 | BAM32 | HDAPP1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence SALCISPEEKTDHK, from the C-Terminus of protein sequence according to AAF14578.1.
Specificity
Human DAPP1. This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.
Applications
- Western blot (0.5 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx 26kDa band in Human Heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.0kDa according to AAF14578.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee