Human CSPG5 / Neuroglycan C

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

CHO cell-derived rhNeuroglycan C extracellular domain.

Extracellular Domain

Recognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.

• ICC (10 µg/ml)
• Western blot (1 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Applications:Suitable for use in ELISA, Immunocytochemistry and WesternBlot. Other Applications not tested.Recommended Dilution:Direct ELISA: 0.5-1ug/ml. The detection limit for recombinanthuman Neuroglycan C is approximately 0.3ng/well.Western Blot: 1ug/mlImmunocytochemistry: 10ug/ml was used to detect NeuroglycanC in sections of adult mouse cerebellum. Cells were fixed withPBS containing 4% paraformaldehyde for 20 minutes at roomtemperature and blocked with PBS containing 10% normaldonkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutesat RT, After blocking, cells were incubated with dilutedLS-C123249 for 3 hours at RT followed by NorthernLights 557donkey anti-sheep lgG at RT for 1 hour. Between each step, cellswere washed with PBS containing 0.1% BSA.Optimal dilutions to be determined by the researcher

Lyophilized from Sterile Solution PBS, pH 7.4, 5% Trehalose

Reconstitute with 500ul sterile PBS

Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95196 NCBI: NM_006574 NP_006565.2