Target
Human CRYAB / Alpha B Crystallin
Synonyms
CRYAB | Alpha-crystallin B chain | CRYA2 | Crystallin, alpha B | CTPP2 | Heat shock protein beta-5 | HSPB5 | Alpha B Crystallin | Alpha(B)-crystallin | Rosenthal fiber component | Heat-shock 20 kD like-protein
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Sheep
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Human synthetic peptide corresponding to amino acids 54-64 and containing phosphorylated serine 59. This peptide is conserved among mouse, rat and bovine.
Specificity
This antibody detects an ~22kD protein, corresponding to the apparent molecular mass of phosphorylated alpha-B-crystallin on SDS-PAGE immunoblots, in samples from mouse, rat, bovine, canine, porcine and sheep. There is no reactivity with non-phosphorylated alpha B-crystallin. This antibody may cross react with beta crystallin.
Applications
- Western blot
- Immunoprecipitation (12.5 µg/ml)
Usage
Western Blot (Colorimetric): 2 ug/mL. Immunoprecipitation: 12.5 ug/mL. Positive control: Alpha Crystallin Protein.
Presentation
PBS, pH 7.0, 3% BSA, 0.1% sodium azide, 50% glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRYAB / Alpha B Crystallin