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Antibodies > CRP / C-Reactive Protein > LS-C661942
 
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Catalog Number Size Price
LS-C661942-10 10 µg $318 
LS-C661942-100 100 µg $470 
CRP / C-Reactive Protein Antibody - Immunohistochemistry - Anti-Crp/C Reactive Protein Picoband Antibody
CRP / C-Reactive Protein Antibody - Immunohistochemistry - Anti-Crp/C Reactive Protein Picoband Antibody
CRP / C-Reactive Protein Antibody - Western blot analysis of Crp using anti-Crp antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crp antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Crp at approximately 25KD. The expected band size for Crp is at 25KD.
CRP / C-Reactive Protein Antibody - Western blot - Anti-Crp/C Reactive Protein Picoband Antibody
CRP / C-Reactive Protein Antibody - Immunohistochemistry - Anti-Crp/C Reactive Protein Picoband Antibody
CRP / C-Reactive Protein Antibody - Immunohistochemistry - Anti-Crp/C Reactive Protein Picoband Antibody
CRP / C-Reactive Protein Antibody - Western blot analysis of Crp using anti-Crp antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crp antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Crp at approximately 25KD. The expected band size for Crp is at 25KD.
CRP / C-Reactive Protein Antibody - Western blot - Anti-Crp/C Reactive Protein Picoband Antibody
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Polyclonal Rabbit anti‑Mouse CRP / C‑Reactive Protein Antibody (IHC, WB) LS‑C661942

Polyclonal Rabbit anti‑Mouse CRP / C‑Reactive Protein Antibody (IHC, WB) LS‑C661942

Submit Review LSBio Guarantee
Antibody:
CRP / C-Reactive Protein Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661942-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CRP / C-Reactive Protein Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Reactive Protein antibody LS-C661942 is an unconjugated rabbit polyclonal antibody to mouse C-Reactive Protein (CRP). Validated for ELISA, IHC and WB.
Target
Mouse CRP / C-Reactive Protein
Synonyms
CRP | C-reactive protein | Pentraxin 1 | PTX1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Crp recombinant protein (Position: H20-S225). Mouse Crp shares 71.9% and 72.7% amino acid (aa) sequence identity with human and rat Crp, respectively.
Specificity
Found in plasma.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRP / C-Reactive Protein
P02741 NM_000567 NP_000558.2

Publications (0)

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See all CRP / C-Reactive Protein Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
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