Target
Human CREB1 / CREB
Synonyms
CREB1 | CREB-1 | Transactivator protein | CREB
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Specificity
Recognizes phospho-CREB (Ser133), Mr 43kD. Species cross-reactivity: Rat and mouse. Broad species cross-reactivity expected, based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 1:1000 to 1:4000. Detects phospho-CREB (Ser133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Lysates from untreated (Lanes 1 and 3) and forskolin treated (Lanes 2 and 4) PC-12 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (Ser133) (1:4000 dilution, Lanes 1 and 2) or anti-CREB (1:2000 dilution, Lanes 3 and 4). Proteins were visualized using a goat antimouse (Lanes 1and 2) or goat antirabbit (Lanes 3 and 4) secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee