Rat CREB1 / CREB

Rabbit

Rat, Human, Mouse, Bovine, Dog, Pig, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic phosphopeptide corresponding to residues 123-136 [KRREILSRRP(pS)Y-RK] of rat CREB (cAMP response element binding protein) coupled to keyhole limpet hemocyanin (KLH). Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Dog, Pig, Xenopus, Zebrafish (100%).

pSer133

Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kD proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide. Species cross-reactivity: Rat, mouse and human.

• IHC
• IHC - Paraffin
• Western blot (1:500)
• Immunoprecipitation
• Gel shift

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (formalin-fixed, paraffin). Western Blot: 1:500. Detected phosphorylated CREB in RIPA lysates from mouse 3T3 cells treated with 50 mM forskolin. PC-12 and forskolin treated PC-12 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Gel Shift: This antibody has been shown to gel shift. Immunoprecipitation: 20 ul immunoprecipitates phosphorylated CREB from 500 ug of rat PC-12 cells treated with 50 uM forskolin. Immunohistochemistry: 1:500 dilution detects phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.

0.1 M Tris-glycine, pH 7.4. 150 mM sodium chloride, 0.05% sodium azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16220 NCBI: NM_134442 NP_004370.1