Target
Pig CPB / Carboxypeptidase B
Synonyms
CPB1 | Carboxypeptidase B | Carboxypeptidase B1 (tissue) | CPB | Pancreatic carboxypeptidase B | PASP | Procarboxypeptidase B | Protaminase | Pancreas-specific protein | PCPB | Tissue carboxypeptidase B
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Carboxypeptidase B [Porcine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
Usage
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CPB / Carboxypeptidase B