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Catalog Number	Size	Price
LS-C662961-10	10 µg	$318
LS-C662961-100	100 µg	$470

COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody

COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody

COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.

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Polyclonal Rabbit anti‑Human COXIV / COX4 Antibody (IHC, WB) LS‑C662961

Name: Anti-COXIV / COX4 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662961-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

COXIV / COX4 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662961-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

COXIV / COX4 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

COX4 antibody LS-C662961 is an unconjugated rabbit polyclonal antibody to human COX4 (COXIV). Validated for ELISA, IHC and WB.

Target

Human COXIV / COX4

Synonyms

COX4I1 | COX4-1 | COX4 | COX IV-1 | COXIV

See All COXIV / COX4 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived human COX IV recombinant protein (Position: Q59-K169).

Specificity

Ubiquitous.

Applications

IHC
IHC - Paraffin
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About COXIV / COX4

Uniprot: P13073 NCBI: NM_001861 NP_001852.1