Target
Human Complement C1QA
Synonyms
C1QA | Complement C1QA
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Purified human complement C1q.
Specificity
Recognizes the C-chain of complement C1q (25kD in SDS-PAGE). Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders. Detects C1q in serum, in serum-C1 and within immune complexes.
Applications
- Western blot (1:100)
- ELISA
- Functional Assay
Usage
Suitable for use in ELISA, Western Blot and Inhibition experiments (e. g. hemolytic activity). Western Blot: 1:100 (ECL method); Incubation Time 1hr at RT. Inhibition of hemolytic activity: 0.85 ug/ml inhibits 50% of hemolytic activity in the fluid phase. With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5 ug/ml.
Presentation
Lyophilized from PBS, pH 7.4, 0.09% Sodium Azide, 0.5% BSA
Reconstitution
Reconstitute with 1ml sterile ddH2O.
Storage
Reconstitute, aliquot, and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee