Human Collagen VII

Mouse

Human, Guinea pig, Sheep (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

LH7.2

Unconjugated

Ascites

Unmodified

Full length native protein (purified) (Human). Insoluble fractions prepared from neonatal foreskin epidermal cells. Epitope: Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i. e. the non-helical C-Terminus of the Type VII collagen dimer.

C-Terminus

Recognizes human tissue Type VII collagen. Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e., the non-helical carboxy terminal region of the Type VII collagen dimer. It is also reactive in immunoblotting against intact tissue Type VII collagen (250 kDa), but once the terminal regions are completely digested by pepsin, this reactivity is completely lost. No reactivity against collagen types I, II, III, IV, V, pepsin-digested collagen type VII, laminin and fibronectin. Species Crossreactivity: Basement membrane zone of stratified squamous epithelia of human, bovine, porcine, goat, guinea pig, monkey, marmoset and sheep.

• IHC
• IHC - Frozen (1:2000)
• ICC
• Immunofluorescence (1:1000)
• Western blot
• ELISA

Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Immunofluorescence: 1:1000. Immunohistochemistry: Frozen sections fixed in acetone.

15 mM Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.