Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Collagen Type II from adult human knee cartilage and bovine nasal cartilage. Collagen Type II was extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein.
Specificity
Recognizes Type II collagens. Species cross-reactivity: most mammals. Cross-reactivity with Type I, III, IV, V or VI collagens is negligible. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Western blot
- Immunoprecipitation
- ELISA
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Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ABTS. Western Blot (Non-denaturing, non-dissociating): 1:2500-1:5000. Immunohistochemistry (frozen tissue sections): 1:25-1:100. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- fixed, paraffin embedded tissues.
Presentation
0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide, 40% glycerol. No stabilizing proteins added.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee