Target
Human COL4A6 / Collagen IV
Synonyms
COL4A6 | Collagen, type IV, alpha 6 | Collagen alpha 6 type IV | Collagen alpha 6(iv) | Collagen alpha-6(IV) chain | CXDELq22.3 | DELXq22.3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PSPEFETETLHNKES, from the internal region of the protein sequence according to NP_001838.2NP_378667.1.
Specificity
Human COL4A6. This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL4A6 / Collagen IV