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Catalog Number	Size	Price
LS-C353940-30	30 µl	$255
LS-C353940-50	50 µl	$271
LS-C353940-100	100 µl (1 mg/ml)	$299
LS-C353940-200	200 µl	$351

COL4A5 / Collagen IV Alpha5 Antibody - Immunohistochemical analysis of Collagen 4 alpha 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

COL4A5 / Collagen IV Alpha5 Antibody - Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

COL4A5 / Collagen IV Alpha5 Antibody - Western blot analysis of Collagen 4 alpha 5 expression in HeLa (A); mouse brain (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human COL4A5 / Collagen IV Alpha5 Antibody (N‑Terminus, IHC, IF, WB) LS‑C353940

Name: Anti-COL4A5 / Collagen IV Alpha5 Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C353940-30
Price: 255.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

COL4A5 / Collagen IV Alpha5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$255

LS-C353940-30

$271

LS-C353940-50

$299

LS-C353940-100

$351

LS-C353940-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

COL4A5 / Collagen IV Alpha5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

Collagen IV Alpha5 antibody LS-C353940 is an unconjugated rabbit polyclonal antibody to Collagen IV Alpha5 (COL4A5) (N-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.

Target

Human COL4A5 / Collagen IV Alpha5

Synonyms

See All COL4A5 / Collagen IV Alpha5 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Collagen 4 alpha 5.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of Collagen 4 alpha 5 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About COL4A5 / Collagen IV Alpha5

Uniprot: P29400 NCBI: NM_000495 NP_000486.1