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Catalog Number	Size	Price
LS-C352027-100	100 µl (1 mg/ml)	$299

COL3A1 / Collagen III Antibody - Immunohistochemical analysis of Collagen 3 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

COL3A1 / Collagen III Antibody - Immunofluorescent analysis of Collagen 3 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

COL3A1 / Collagen III Antibody - Western blot analysis of Collagen 3 alpha 1 expression in A10 (A); HeLa (B); mouse lung (C); mouse liver (D); rat lung (E) whole cell lysates.

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Polyclonal Rabbit anti‑Human COL3A1 / Collagen III Antibody (N‑Terminus, IHC, IF, WB) LS‑C352027

Name: Anti-COL3A1 / Collagen III Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C352027-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

COL3A1 / Collagen III Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$299

LS-C352027-100

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(800) 227-6666

For Research Use Only

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Overview

Antibody:

COL3A1 / Collagen III Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Collagen III antibody LS-C352027 is an unconjugated rabbit polyclonal antibody to Collagen III (COL3A1) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.

Target

Human COL3A1 / Collagen III

Synonyms

See All COL3A1 / Collagen III Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Collagen 3 alpha 1.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of Collagen 3 alpha 1 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About COL3A1 / Collagen III

Uniprot: P02461 NCBI: NM_000090 NP_000081.1