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Catalog Number	Size	Price
LS-C313426-10	10 µg	$318
LS-C313426-100	100 µg (0.5 mg/ml)	$470

CNTF Antibody - Immunohistochemistry - Anti-CNTF Picoband Antibody

CNTF Antibody - Western blot analysis of CNTF using anti-CNTF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CNTF at approximately 29KD. The expected band size for CNTF is at 23KD.

CNTF Antibody - Western blot - Anti-CNTF Picoband Antibody

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Polyclonal Rabbit anti‑Mouse CNTF Antibody (aa2‑198, IHC, WB) LS‑C313426

Name: Anti-CNTF Antibody | Rabbit anti-Mouse Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313426-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CNTF Rabbit anti-Mouse Polyclonal (aa2-198) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C313426-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CNTF Rabbit anti-Mouse Polyclonal (aa2-198) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CNTF antibody LS-C313426 is an unconjugated rabbit polyclonal antibody to CNTF (aa2-198) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.

Target

Mouse CNTF

Synonyms

CNTF | Ciliary neurotrophic factor | HCNTF

See All CNTF Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived mouse CNTF recombinant protein (Position: A2-M198). Mouse CNTF shares 83% and 95% amino acid (aa) sequences identity with human and rat CNTF, respectively.

Epitope

aa2-198

Specificity

Nervous system.

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CNTF

Uniprot: P26441 NCBI: NM_000614 NP_000605.1