Target
Human CNR1 / CB1
Synonyms
CNR1 | CB-R | CB1 receptor | CB1A | CB1R | Cannabinoid receptor 1 | Cannabinoid receptor CB1 | Cannabinoid Receptor I | CB1 | CB1K5 | CANN6 | Cannabinoid receptor 1 (brain) | CNR | Cannabinoid CB(1) receptor | CB1 cannabinoid receptor | Central cannabinoid receptor
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-NITEFYNKSLSSFKE, from the internal region (near N-Terminus) of the protein sequence according to NP_057167.2NP_149421.2.
Specificity
Human CNR1 / CB1. This antibody is expected to recognize both reported isoforms (NP_057167.2; NP_149421.2). Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70+55kD bands observed in Rat Brain lysates (calculated MW of 52.9kD according to Human NP_057167.2 and 52.8kD according to Rat NP_036916.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee