Target
Human CML / BCR
Synonyms
BCR | Acute lymphocytic leukemia | ALL | BCR1 | BCR/FGFR1 chimera protein | Breakpoint cluster region | Chronic myeloid leukemia | D22S11 | CML | D22S662 | Philadelphia chromosome | PHL | FGFR1/BCR chimera protein
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human Bcr around the phosphorylation site of Tyrosine 177.
Specificity
Phospho-Bcr (Tyr177) Antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177.
Applications
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee