Human CFTR

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Fusion Protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395.

aa1365-1395

Recognizes CFTR, Mr 170kD. Species cross-reactivity: Human.

• IHC
• Western blot
• Immunoprecipitation
• ELISA

Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation: Reported to immunoprecipitate CFTR.1. Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1. Western Blot Analysis: 0.5-2 ug/ml detects CFTR from 20-50 ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37?C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13569 NCBI: NM_000492 NP_000483.3