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Catalog Number Size Price
LS-C148270-50 50 µl $386 
CFLAR / FLIP Antibody - IHC of FLIP in tissues cores from formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural edema brain tissue. Reactive astrocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-Eos in counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in tissues cores from formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural edema brain tissue. Reactive astrocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-Eos in counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.
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Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (aa75‑92, IHC, WB) LS‑C148270

Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (aa75‑92, IHC, WB) LS‑C148270

Antibody:
CFLAR / FLIP Rabbit anti-Human Polyclonal (aa75-92) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$386
LS-C148270-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CFLAR / FLIP Rabbit anti-Human Polyclonal (aa75-92) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
FLIP antibody LS-C148270 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) (aa75-92) from human. It is reactive with human and monkey. Validated for IHC, IP and WB.
Target
Human CFLAR / FLIP
Synonyms
CFLAR | C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP, GenBank no. gi|12643547. This amino acid sequence is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www. ncbi. nlm. nih. gov/blast /. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (94%); Marmoset (88%); Panda (82%); Mouse (76%); Rat (71%).
Epitope
aa75-92
Specificity
The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFLAR / FLIP
O15519 NM_003879 NP_003870.4

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Requested From: United States
Date Requested: 12/21/2024