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Catalog Number	Size	Price
LS-C313470-10	10 µg (200 µg/ml)	$318
LS-C313470-100	100 µg (200 µg/ml)	$470

CEACAM5 / CD66e Antibody - Western blot analysis of CEA using anti-CEA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.

CEACAM5 / CD66e Antibody - WB of CEACAM5 / CD66e antibody. Lane 1: Recombinant Human CEA Protein 5ng. Lane 2: Recombinant Human CEA Protein 2.5ng. Lane 3: Recombinant Human CEA Protein 1.25ng. Lane 4: SW620 Cell Lysate.

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Polyclonal Rabbit anti‑Human CEACAM5 / CD66e Antibody (IHC, WB) LS‑C313470

Name: Anti-CEACAM5 / CD66e Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313470-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Note: This antibody replaces LS-C388243

Antibody:

CEACAM5 / CD66e Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C313470-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CEACAM5 / CD66e Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD66e antibody LS-C313470 is an unconjugated rabbit polyclonal antibody to human CD66e (CEACAM5). Validated for ELISA, IHC and WB.

Target

Human CEACAM5 / CD66e

Synonyms

See All CEACAM5 / CD66e Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

Insect cells-derived human CEA recombinant protein(Position: full).

Specificity

Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Applications

IHC
IHC - Paraffin
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CEACAM5 / CD66e

Uniprot: P06731 NCBI: NM_004363 NP_004354.3