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Catalog Number	Size	Price
LS-C354630-100	100 µl (1 mg/ml)	$315

CDKN1B / p27 Kip1 Antibody - Immunohistochemical analysis of p27 Kip1 (pT187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w

CDKN1B / p27 Kip1 Antibody - Immunofluorescent analysis of p27 Kip1 (pT187) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CDKN1B / p27 Kip1 Antibody - Western blot analysis of p27 Kip1 (pT187) expression in HeLa (A); MCF7 (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (phospho‑Thr187, IHC, IF, WB) LS‑C354630

Name: Anti-CDKN1B / p27 Kip1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C354630-100
Price: 315.0000 USD
Availability: InStock

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Antibody:

CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pThr187) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Bovine, Dog

Format:

Unconjugated, Unmodified

$315

LS-C354630-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pThr187) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Bovine, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

P27 Kip1 antibody LS-C354630 is an unconjugated rabbit polyclonal antibody to p27 Kip1 (CDKN1B) (pThr187) from human. It is reactive with human, mouse, bovine and other species. Validated for ICC, IF, IHC and WB.

Target

Human CDKN1B / p27 Kip1

Synonyms

See All CDKN1B / p27 Kip1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Bovine, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human p27 Kip1 (pT187).

Epitope

pThr187

Specificity

Recognizes endogenous levels of p27 Kip1 (pT187) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CDKN1B / p27 Kip1

Uniprot: P46527 NCBI: NM_004064 NP_004055.1