Mouse CDKN1B / p27 Kip1

Mouse

Mouse, Human, Monkey, Rat (tested or 100% immunogen sequence identity)

IgG Monoclonal

DCS-72.F6 + KIP1/769

Unconjugated

Protein G purified

Azide-free, BSA-free

Mouse recombinant protein (DCS-72. F6) and recombinant human protein (KIP1/769) were used as the immunogen for the p27 antibody cocktail.

Mouse SSSCA1 / p27

• IHC
• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Optimal dilution of the p27 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46527 NCBI: NM_004064 NP_004055.1