Human CDKN1A / WAF1 / p21

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

WA-1

Unconjugated

Protein A purified

Unmodified

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence (1:250)
• Western blot (1:2000 - 1:10000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

This antibody is suitable for immunohistochemistry, ELISA and immunoblotting. The antibody detects a 21 kD band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21 WAF1 and not p21 complexes with other proteins. High level expression of p21 WAF1 requires induction of wild-type p53 expression (e. g. via exposure to DNA damaging agents).

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P38936 NCBI: NM_078467 NP_000380.1