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| Catalog Number | Size | Price |
|---|---|---|
| LS-C17111-50 | 50 µg | $890 |
Monoclonal Mouse anti‑Human CDKN1A / WAF1 / p21 Antibody (aa58‑77, IHC, IF, WB) LS‑C17111
Monoclonal Mouse anti‑Human CDKN1A / WAF1 / p21 Antibody (aa58‑77, IHC, IF, WB) LS‑C17111
Antibody:
CDKN1A / WAF1 / p21 Mouse anti-Human Monoclonal (aa58-77) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CDKN1A / WAF1 / p21 Mouse anti-Human Monoclonal (aa58-77) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
P21 antibody LS-C17111 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1) (aa58-77). Validated for Flow, IF, IHC, IP and WB.
Target
Human CDKN1A / WAF1 / p21
Synonyms
CDKN1A | CDKN1 | CIP1 | CDK-interacting protein 1 | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Full length recombinant human p21Cip1/WAF1.
Epitope
aa58-77
Specificity
Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence (1 - 5 µg/ml)
- Western blot
- Immunoprecipitation
- Flow Cytometry (2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Flow Cytometry: 2 ug/ml. Western Blot (chemiluminescence): 0.1-1 ug/ml. Immunohistochemistry (frozen/paraffin): 5 ug/ml. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 ug/IP reaction. Immunoprecipitates p21 but not associated proteins.
Presentation
Supplied s a liquid in PBS, pH 7.4, 0.1% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDKN1A / WAF1 / p21
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Featured Products
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Species:
Human, Chimpanzee, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/16/2025
Date Requested: 3/16/2025
