Human CDH6 / K Cadherin

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

NS0-derived rhCadherin-6 extracellular domain.

Extracellular Domain

Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5% cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.

• ICC (10 µg/ml)
• Western blot
• Flow Cytometry (50 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow Cytometry: 50 ug/ml Add 10 ul of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute in sterile PBS.

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P55285 NCBI: NM_004932 P55285