Human CDH13 / Cadherin 13

Rat

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

392411

Unconjugated

Protein G purified

Unmodified

NS0-derived recombinant human Cadherin-13 (aa23-692) Accession # P55290.

aa23-692

Recognizes human Cadherin-13. Does not cross-react with rhECAD, rhNCAD, rhPCAD, rhVECAD, rhCAD-8, rhCAD-11, rhCAD-12 or rhCAD-17.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Flow Cytometry and ELISA. Direct ELISA: 0.5-1 ug/ml, detection limit: 7 ng/well. Western Blot: 1-2 ug/ml, detects 5 ng/lane under non-reducing conditions. Not recommended for use under reducing conditions. Flow Cytometry: 25 ug/ml. Add 10 ul of the diluted. solution to 1-2.5 x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Positive control: NCI-H460 human large cell lung cancer cells.

Lyophilized from PBS, 5% Trehalose

Reconstitute in 200ul sterile PBS.

Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P55290 NCBI: NM_001257 NP_001248.1