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Antibodies > CDC25C > LS-C358446
 
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Catalog Number Size Price
LS-C358446-100 100 µl (1 mg/ml) $315 
CDC25C Antibody - Immunohistochemical analysis of CDC25C (pS216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CDC25C Antibody - Immunofluorescent analysis of CDC25C (pS216) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CDC25C Antibody - Western blot analysis of CDC25C (pS216) expression in HEK293T (A); A549 (B) whole cell lysates.
CDC25C Antibody - Immunohistochemical analysis of CDC25C (pS216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CDC25C Antibody - Immunofluorescent analysis of CDC25C (pS216) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CDC25C Antibody - Western blot analysis of CDC25C (pS216) expression in HEK293T (A); A549 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human CDC25C Antibody (phospho‑Ser216, IHC, IF, WB) LS‑C358446

Polyclonal Rabbit anti‑Human CDC25C Antibody (phospho‑Ser216, IHC, IF, WB) LS‑C358446

Submit Review LSBio Guarantee
Antibody:
CDC25C Rabbit anti-Human Polyclonal (pSer216) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C358446-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDC25C Rabbit anti-Human Polyclonal (pSer216) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25C antibody LS-C358446 is an unconjugated rabbit polyclonal antibody to CDC25C (pSer216) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human CDC25C
Synonyms
CDC25C | CDC25 | CDC25C phosphatase | CDC25Hu3 | Cell division cycle 25C | Phosphotyrosine phosphatase | PPP1R60 | M-phase inducer phosphatase 3 | Mitosis inducer CDC25
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human CDC25C (pS216).
Epitope
pSer216
Specificity
Recognizes endogenous levels of CDC25C (pS216) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25C
P30307 NM_001790 NP_001781.2

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Featured Products

CDC25C Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25C Antibody (phospho‑Ser216) LS‑C808495
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CDC25C Antibody - Immunofluorescence analysis of U2OS cells.
CDC25C Antibody LS‑C331630
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation
See all CDC25C Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/10/2025
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