Target
Human CDC20
Synonyms
CDC20 | BA276H19.3 | CDC20A | p55CDC
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Specificity
Reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Applications
- IHC
- Western blot (1:500 - 1:1000)
- Immunoprecipitation
- ELISA (1:2000 - 1:10000)
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Usage
This antibody reacts with human CDC20 (fizzy) by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kD band corresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressing CDC20 can be used as a positive control. Researchers should determine optimal titers for other applications.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee