Target
Human CD96 / TACTILE
Synonyms
CD96 | CD96 molecule | CD96 antigen | Cell surface antigen CD96 | T-cell surface protein tactile | TACTILE | T-Cell Activation Antigen, Increased Late Expression
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KYTCIQEPNESD, from the C-Terminus of protein sequence according to NP_937839.1NP_005807.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_937839.1; NP_005807.1).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 100+75kDa bands in Jurkat lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.6kDa according to NP_937839.1. The 100kDa and 75kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee