Mouse CD80

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

RM80

FITC. Also available Unconjugated or conjugated with RPE.

Protein G purified

Unmodified

BCL1 cells expressing CD80

Recognizes mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

• Flow Cytometry (1:1)
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

PBS, 0.09% Sodium Azide, 1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P33681 NCBI: NM_005191 NP_005182.1