Human CD80

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG,l Recombinant Monoclonal

IDEC-114

Unconjugated

Protein A affinity purified

Unmodified

Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)

Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.

• IHC (5 µg/ml)
• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA
• Blocking
• Functional Assay

This rabbit recombinant antibody is also available in a humanized form, LS-C821451. Applications should be user optimized. For IHC-P the suggested antigen retrieval is HIER using citrate buffer pH5 held at 90-98oC for 15 min, then allow to cool to room temp.

PBS, 0.02% Proclin 300

Store undiluted at 4°C for up to 3 months. Aliquot and store at -20°C for long term storage.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P33681 NCBI: NM_005191 NP_005182.1