Human CD74 / CLIP

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

BU45

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

The B lymphoblastoid cell line HFB1.

Human CD74

• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This antibody recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74. The CD74 antibody epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. CD74 antibody has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). ELISA: Order BSA-free format for coating. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04233 NCBI: NM_004355 NP_004346.1