Human CD63

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

AHN16.1/46-4-5

Phycoerythrin

Protein A purified

Unmodified

Human eosinophils

Recognizes the CD63 molecule of about 50kD.

• Flow Cytometry

Suitable for use in Flow Cytometry. FC: Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells). 5x10e5 cultured HPB-MLT cells were washed and incubated 45mins. on ice with 80ul of LS-C35719 at a 1:50 dilution factor(20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.70 log10fluorescent units when compared to a mouse IgG1/PE negativecontrol at a similar concentration. Binding was blocked when cellswere pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD63antibody (Cat. #LS-C35716). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide.

Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing PE conjugates will result in a substantial loss of enzymatic activity.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08962 NCBI: NM_001780 NP_001771.1