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Catalog Number Size Price
LS-C662675-10 10 µg $318 
LS-C662675-100 100 µg $470 
cd59a Antibody - Immunohistochemistry - Anti-CD59  antibody on mouse thymus
cd59a Antibody - Immunohistochemistry - Anti-CD59  antibody on mouse spleen
cd59a Antibody - Western blot analysis of CD59 using anti-CD59 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD59 at approximately 20KD. The expected band size for CD59 is at 14KD.
cd59a Antibody - Immunohistochemistry - Anti-CD59  antibody on mouse thymus
cd59a Antibody - Immunohistochemistry - Anti-CD59  antibody on mouse spleen
cd59a Antibody - Western blot analysis of CD59 using anti-CD59 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD59 at approximately 20KD. The expected band size for CD59 is at 14KD.
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Polyclonal Rabbit anti‑Mouse cd59a Antibody (IHC, WB) LS‑C662675

Polyclonal Rabbit anti‑Mouse cd59a Antibody (IHC, WB) LS‑C662675

Antibody:
cd59a Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662675-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
cd59a Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Cd59a antibody LS-C662675 is an unconjugated rabbit polyclonal antibody to mouse cd59a. Validated for IHC and WB.
Target
Mouse cd59a
Synonyms
cd59a
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived mouse CD59 recombinant protein (Position: L24-S96).
Applications
  • IHC - Paraffin (2 µg/ml)
  • Western blot (0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About cd59a

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/19/2024