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Antibodies > CD55 > LS-C407699
 
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Catalog Number Size Price
LS-C407699-10 10 µg $318 
LS-C407699-100 100 µg $470 
CD55 Antibody - CD55 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
CD55 Antibody - Western blot analysis of CD55 using anti-CD55 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD55 at approximately 60KD. The expected band size for CD55 is at 41KD.
CD55 Antibody - CD55 antibody Western blot. All lanes: Anti CD55 at 0.5 ug/ml. WB: K562 Whole Cell Lysate at 40 ug. Predicted band size: 41 kD. Observed band size: 41 kD.
CD55 Antibody - Flow Cytometry analysis of SiHa cells using anti-CD55 antibody. Overlay histogram showing SiHa cells stained with anti-CD55 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD55 Antibody - Flow Cytometry analysis of PC-3 cells using anti-CD55 antibody. Overlay histogram showing PC-3 cells stained with anti-CD55 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD55 Antibody - CD55 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
CD55 Antibody - Western blot analysis of CD55 using anti-CD55 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD55 at approximately 60KD. The expected band size for CD55 is at 41KD.
CD55 Antibody - CD55 antibody Western blot. All lanes: Anti CD55 at 0.5 ug/ml. WB: K562 Whole Cell Lysate at 40 ug. Predicted band size: 41 kD. Observed band size: 41 kD.
CD55 Antibody - Flow Cytometry analysis of SiHa cells using anti-CD55 antibody. Overlay histogram showing SiHa cells stained with anti-CD55 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD55 Antibody - Flow Cytometry analysis of PC-3 cells using anti-CD55 antibody. Overlay histogram showing PC-3 cells stained with anti-CD55 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CD55 Antibody (aa35‑347, IHC, WB) LS‑C407699

Polyclonal Rabbit anti‑Human CD55 Antibody (aa35‑347, IHC, WB) LS‑C407699

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Antibody:
CD55 Rabbit anti-Human Polyclonal (aa35-347) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407699-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CD55 Rabbit anti-Human Polyclonal (aa35-347) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CD55 antibody LS-C407699 is an unconjugated rabbit polyclonal antibody to CD55 (aa35-347) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human CD55
Synonyms
CD55 | CD55 antigen | Decay-accelerating factor | TC | CR | CROM | DAF
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
Epitope
aa35-347
Specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD55
P08174 NM_000574 NP_000565.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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