Human CD47

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

IAP/964 + B6H12.2

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Recombinant human protein (IAP/964) and intact CD47 purified from placenta (B6H12.2) were used as the immunogen for the CD47 antibody cocktail.

Human CD47

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Optimal dilution of the CD47 antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q08722 NCBI: NM_001777 NP_001768.1