Human CD45RO

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

190-2F2.5

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Lenti lectin purified glycoproteins obtained from human peripheral blood lymphocytes and from a CLL (B cell) were used as immunogen for this CD45RO antibody.

Human CD45RO

• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.