Mouse CD45R

Rat

Mouse (tested or 100% immunogen sequence identity)

IgM Monoclonal

RA3-3A1/6.1

Unconjugated. Also available conjugated with Biotin, FITC.

Size exclusion chromatography

Unmodified

RAW112 cells.

Antibody RA3-3A1 binds to an extracellular epitope of B220 (CD45R), the 220 kD variant of the T200, CD45 molecule present on B cells and B cell precursors.

• Flow Cytometry

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20ul of 300 ug/ml murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-220 antibody diluted to 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 11.3% sub population of the cells stained positive with a mean shift of 1.5 log10 fluorescent units when compared to background.

50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/ml Gentamicin sulfate

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.