Human CD44

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

BU52

Unconjugated

Protein A purified

Fab'2. Also available Unmodified.

Human peripheral myeloma cells

Epitope 1 of the CD44 molecule.

• Western blot
• Immunoprecipitation
• Flow Cytometry

Five x 105 Ficoll prepared human peripheral blood mononuclear cells were incubated 45 minutes on ice with 80 ul of anti-CD44 F(ab')2 at 5 ug/ml. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 2.13 log10 fluorescent units when compared to a Mouse IgG1 F(ab')2 at a similar concentration. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Sterile Solution 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, No preservatives added

Store at 4°C. Do not freeze. Open under aseptic conditions.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16070 NCBI: NM_000610 NP_000601.3