Human CD34

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Recombinant Monoclonal

HPCA1/2598R

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human HPCA1 protein. Antigen Molecular Weight: 90-110 kDa

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa , which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

• IHC - Paraffin (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 min at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P28906 NCBI: NM_001773 NP_001764.1