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Catalog Number Size Price
LS-C797116-100 100 µg $511 
CD32A Antibody - IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - Anti-FCGR2A mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
CD32A Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
CD32A Antibody - Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CD32A Antibody - IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
CD32A Antibody - Anti-FCGR2A mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
CD32A Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
CD32A Antibody - Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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Monoclonal Mouse anti‑Human CD32A Antibody (clone OTI9C6, Carrier‑free, IHC, IF, WB) LS‑C797116

Monoclonal Mouse anti‑Human CD32A Antibody (clone OTI9C6, Carrier‑free, IHC, IF, WB) LS‑C797116

Antibody:
CD32A Mouse anti-Human Monoclonal (Carrier-free) (OTI9C6) Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$511
LS-C797116-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD32A Mouse anti-Human Monoclonal (Carrier-free) (OTI9C6) Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
CD32A antibody LS-C797116 is an unconjugated mouse monoclonal antibody to human CD32A. Validated for IF, IHC, IP and WB.
Target
Human CD32A
Synonyms
FCGR2A | CD32 | CD32 antigen | CDw32 | FCG2 | FcGR | FCGR2A1 | FcRII-a | Fc-gamma RII-a | Fc-gamma-RIIa | FcRII-c | IgG Fc receptor II-a | Igg fc receptor ii-c | Fc-gamma rii-c | FCGR2 | Igg fc receptors ii-c | CD32A | IGFR2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI9C6
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Applications
  • IHC (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:1000)
  • Immunoprecipitation (4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD32A
P12318 NM_021642 NP_067674.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024