Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C58677-0.1 | 0.1 mg (1 mg/ml) | $395 |
Monoclonal Mouse anti‑Rat CD3 Antibody (clone 1F4, IHC) LS‑C58677
Monoclonal Mouse anti‑Rat CD3 Antibody (clone 1F4, IHC) LS‑C58677
Note: This antibody replaces LS-C58672
Antibody:
CD3 Mouse anti-Rat Monoclonal (1F4) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD3 Mouse anti-Rat Monoclonal (1F4) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Specifications
Description
CD3 antibody LS-C58677 is an unconjugated mouse monoclonal antibody to rat CD3. Validated for Flow, IHC and IP.
Host
Mouse
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgM
Monoclonal
Clone
1F4
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
Specificity
Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
Applications
- IHC
- IHC - Paraffin (1:10)
- IHC - Frozen (1:10 - 1:25)
- Immunoprecipitation
- Flow Cytometry (1:10 - 1:25)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Frozen, Flow Cytometry, Functional Assay
Species:
Human
Applications:
Western blot, Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
