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Catalog Number	Size	Price
LS-C407903-10	10 µg	$318
LS-C407903-100	100 µg	$470

CD22 Antibody - CD22 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.

CD22 Antibody - CD22 antibody Western blot. All lanes: Anti CD22 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 95 kD. Observed band size: 95 kD.

CD22 Antibody - Flow Cytometry analysis of Raji cells using anti-CD22 antibody. Overlay histogram showing Raji cells stained with anti-CD22 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human CD22 Antibody (aa696‑724, IHC, WB) LS‑C407903

Name: Anti-CD22 Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407903-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CD22 Rabbit anti-Human Polyclonal (aa696-724) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C407903-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD22 Rabbit anti-Human Polyclonal (aa696-724) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD22 antibody LS-C407903 is an unconjugated rabbit polyclonal antibody to human CD22 (aa696-724). Validated for IHC and WB.

Target

Human CD22

Synonyms

See All CD22 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human CD22 (696-724 aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.

Epitope

aa696-724

Specificity

B-lymphocytes.

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD22

Uniprot: P20273 NCBI: NM_001771 NP_001762.2