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Catalog Number	Size	Price
LS-C800179-100	100 µg	$539

CD2 Antibody - IHC staining of FFPE human spleen using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.

CD2 Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue anti-CD2 clone UMAB6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500).

CD2 Antibody - Immunohistochemical staining of paraffin-embedded mouse colon tissue within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)

CD2 Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD2 clone UMAB6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:500).

CD2 Antibody - IHC staining of paraffin-embedded human tonsil using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 of 0.6mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel pH8.7 at 95-100C 30 minutes [do not let boil] or 10 min in pressure cooker. The image shows strong membranous and cytoplasmic staining in >50 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.

CD2 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

CD2 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

CD2 Antibody - Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 8µm.

CD2 Antibody - Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody. (Clone UMAB6). (1:1000)

CD2 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

CD2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. (1:500)

CD2 Antibody - Flow cytometric analysis of living Jurkat cells, using anti-CD2 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)

CD2 Antibody - Flow cytometric analysis of living CCRF-CEM cells, using anti-CD2 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)

CD2 Antibody - Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid Red)/empty vector Blue) using anti-CD2 antibody. Cells incubated with a non-specific antibody. (Green) were used as isotype control. (1:100)

CD2 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody. (Clone UMAB6). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-CD2. (Clone UMAB6) very specifically recognizes Cd2 antigen on OriGene protein microarray chip.

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Monoclonal Mouse anti‑Human CD2 Antibody (clone UMAB6, Carrier‑free, IHC, IF, WB) LS‑C800179

Name: Anti-CD2 Antibody | Mouse anti-Human Monoclonal Carrier-free | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800179-100
Price: 539.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD2 Mouse anti-Human Monoclonal (Carrier-free) (UMAB6) Antibody

Application:

IHC, IF, WB, Flo, PMA

Reactivity:

Human

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

$539

LS-C800179-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD2 Mouse anti-Human Monoclonal (Carrier-free) (UMAB6) Antibody

Application:

IHC, IF, WB, Flo, PMA

Reactivity:

Human

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

Specifications

Description

CD2 antibody LS-C800179 is an unconjugated mouse monoclonal antibody to human CD2. Validated for Flow, IF, IHC, PMA and WB.

Target

Human CD2

Synonyms

See All CD2 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

UMAB6

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Carrier-free. Also available Unmodified.

Immunogen

Protein expressed in 293T cell transfected with human CD2 expression vector.

Specificity

The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.

Applications

IHC
Immunofluorescence
Western blot (1:2000)
Flow Cytometry
Protein Microarray

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

PBS, pH 7.3, 8% Trehalose

Reconstitution

Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD2

Uniprot: P06729 NCBI: NM_001767 NP_001758.2