Human CD19

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

BU12

Allophycocyanin. Also available Unconjugated or conjugated with Biotin, FITC, RPE.

Protein A purified

Unmodified

Human EB-4 Burkitt lymphoma cell line.

CD19 molecule of about 95 kd.

• Flow Cytometry
• Applications tested for the base form of this product only

Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD19/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 2.76 log10 fluorescent units when compared to a buffer control. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD19 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA

Store at 4°C. Do not freeze. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15391 NCBI: NM_001770 NP_001761.3