Human CD119 / IFNGR1

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Recombinant, human Interferon gamma receptor chain 1, NS0-derived, extracellular domain.

Extracellular Domain

Binds to and neutralizes human Interferon gamma receptor 1, 15.756kD. Blocks cell surface human Interferon gamma receptor 1-mediated bioactivity of recombinant human Interferon gamma receptor 1. In direct ELISA and Western Blot, does not cross-react with human Interferon gamma receptor chain2.

• IHC (15 µg/ml)
• ICC
• Western blot (0.1 - 0.2 µg/ml)
• ELISA
• Functional Assay
• Neutralization

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Neutralization of Human IFN-g R1 Bioactivity: The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is ~0.5-2 ug/ml in the presence of 2 ng/ml of rhIFN-g, using a confluent culture of HeLa cells. ELISA (Direct): 0.5-1 ug/ml. The detection limit for rhIFN-g R1 is ~0.3 ng/well. Western Blot: 0.1-0.2 ug/ml. The detection limit for is ~5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 ug/ml. Detects IFN-g R1 in human PBMC's (peripheral blood mononuclear cells) (fluorescence immunocytochemistry).

PBS, pH 7.4, 5% trehalose. No preservative added.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15260 NCBI: NM_000416 NP_000407.1