Target
Mouse CCNE2 / Cyclin E2
Synonyms
CCNE2 | Cyclin E2 | CYCE2 | G1/S-specific cyclin-E2
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:200 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:20000 - 1:85000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee