Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C18811-100	100 µl (80 mg/ml)	$567

CCNB1 / Cyclin B1 Antibody - Western blot analysis using Anti-Cyclin B1 antibody shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10000 of HRP conjugated Goat-a-Rabbit IgG LS-C60865 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results.

CCNB1 / Cyclin B1 Antibody - Western blot analysis using Anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kD corresponding to human Cyclin B1 (arrowhead). Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10000 of HRP conjugated Goat-a-Rabbit IgG.

1 of 2

2 of 2

Polyclonal Rabbit anti‑Human CCNB1 / Cyclin B1 Antibody (IHC, WB) LS‑C18811

Name: Anti-CCNB1 / Cyclin B1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C18811-100
Price: 567.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CCNB1 / Cyclin B1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$567

LS-C18811-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CCNB1 / Cyclin B1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Cyclin B1 antibody LS-C18811 is an unconjugated rabbit polyclonal antibody to Cyclin B1 (CCNB1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB.

Target

Human CCNB1 / Cyclin B1

Synonyms

CCNB1 | CCNB | Cyclin B1 | G2/mitotic-specific cyclin-B1 | G2/mitotic-specific cyclin B1

See All CCNB1 / Cyclin B1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Delipidated and defibrinated

Modifications

Unmodified

Immunogen

Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

Specificity

Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin B1 from other species may also occur.

Applications

IHC
IHC - Paraffin (1:500)
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:100)
ELISA (1:2000 - 1:10000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

This antibody is suitable for use in ELISA, immunoblotting, immunoprecipitation and other immunological methods requiring high titer and specificity. Specific conditions for reactivity and detection of Cyclin 131 should be optimized by the end user. Expect a band approximately 55-66 kD in size corresponding to Cyclin 131 by western blotting in the appropriate cell lysate or extract.

Presentation

Antiserum, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CCNB1 / Cyclin B1

Uniprot: P14635 NCBI: NM_031966 NP_114172.1