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Catalog Number	Size	Price
LS-C407638-10	10 µg	$318
LS-C407638-100	100 µg	$470

CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

CCNA2 / Cyclin A2 Antibody - IF analysis of Cyclin A2 using anti-Cyclin A2 antibody Cyclin A2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Cyclin A2 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody Western blot. All lanes: Anti Cyclin A2 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: HEPG2 Whole Cell Lysate at 40 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 49 kD. Observed band size: 49 kD.

CCNA2 / Cyclin A2 Antibody - Flow Cytometry analysis of U20S cells using anti-Cyclin A2 antibody. Overlay histogram showing U20S cells stained with anti-Cyclin A2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human CCNA2 / Cyclin A2 Antibody (aa10‑168, IHC, WB) LS‑C407638

Name: Anti-CCNA2 / Cyclin A2 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407638-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CCNA2 / Cyclin A2 Rabbit anti-Human Polyclonal (aa10-168) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat, Dog

Format:

Unconjugated, Unmodified

$318

LS-C407638-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CCNA2 / Cyclin A2 Rabbit anti-Human Polyclonal (aa10-168) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

Cyclin A2 antibody LS-C407638 is an unconjugated rabbit polyclonal antibody to Cyclin A2 (CCNA2) (aa10-168) from human. It is reactive with human, rat and dog. Validated for IHC and WB.

Target

Human CCNA2 / Cyclin A2

Synonyms

See All CCNA2 / Cyclin A2 Antibodies

Host

Rabbit

Reactivity

Human, Rat, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.

Epitope

aa10-168

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CCNA2 / Cyclin A2

Uniprot: P20248 NCBI: NM_001237 NP_001228.1