Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C662244-10	10 µg	$318
LS-C662244-100	100 µg	$470

CCN3 / NOV Antibody - IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. NOV/CCN3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NOV/CCN3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CCN3 / NOV Antibody - IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. NOV/CCN3 was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NOV/CCN3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CCN3 / NOV Antibody - IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. NOV/CCN3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NOV/CCN3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CCN3 / NOV Antibody - IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. NOV/CCN3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NOV/CCN3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CCN3 / NOV Antibody - Western blot analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOV/CCN3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOV/CCN3 at approximately 47KD. The expected band size for NOV/CCN3 is at 39KD.

1 of 5

2 of 5

3 of 5

4 of 5

5 of 5

Polyclonal Rabbit anti‑Human CCN3 / NOV Antibody (IHC, WB) LS‑C662244

Name: Anti-CCN3 / NOV Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662244-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CCN3 / NOV Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662244-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CCN3 / NOV Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NOV antibody LS-C662244 is an unconjugated rabbit polyclonal antibody to human NOV (CCN3). Validated for IHC and WB.

Target

Human CCN3 / NOV

Synonyms

See All CCN3 / NOV Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human NOV/CCN3 (334-357 aa HTNCPKNNEAFLQELELKTTRGKM), different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.

Specificity

Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

Applications

IHC
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CCN3 / NOV

Uniprot: P48745 NCBI: NM_002514 NP_002505.1